Analysis of Transcriptional Feedback Strategy for Reducing Interaction in Gene Expression Processes

TitleAnalysis of Transcriptional Feedback Strategy for Reducing Interaction in Gene Expression Processes
Publication TypeJournal Article
Year of Publication2019
AuthorsNuñez S, Garelli F, Picó J, De Battista H
JournalIFAC-PapersOnLine
Volume52
Pagination526 - 531
ISSN2405-8963
Abstract

Advances in genetic manipulation have allowed the overexpression of proteins and insertion of circuits in cells. However, the expected behaviour can be altered by the internal competition for the limited amount of cellular resources. In this work we analyse a feedback strategy based on transcription inhibition that aims to reduce the interaction in a two-protein expression system. The results allow interpreting the effects of negative feedback on the steady-state protein levels and how the realizable protein region is affected by the feedback loop.

DOI10.1016/j.ifacol.2019.06.116
Research Line: 
Control de sistemas y procesos biológicos
Control of biological processes and systems
Control and monitoring of bioprocesses
Control y monitoreo de bioprocesos